Different Dna Extraction Techniques from Brucella melitensis 16M
نویسندگان
چکیده
Detection of Brucella species using nucleic acid techniques requires efficient unbiased DNA extraction procedures. In this study, we compared six protocols for DNA isolation from pure culture of Brucella melitenses 16M and the efficiency of different extraction processes were evaluated using Polymerase Chain Reaction (PCR).These protocols included boiling, the use of CTAB phenol chloroform extraction, TritonX-100, Chelex 100 with or without proteinaseK and commercial kit. There were some differences between the various DNA extraction methods. The CTAB method seems to have the highest genomic DNA yield with the highest quality for DNA techniques compared to other protocols.
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